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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/32799

Title: Anti-inflammatory properties of phenolic compounds and crude extract from Porphyra dentata
Authors: Katarzyna Kazłowska;Todd Hsu;Chia-Chung Hou;Wen-Chin Yang;Guo-Jane Tsai
Contributors: NTOU:Department of Food Science
國立臺灣海洋大學:食品科學系
Keywords: iNOS;NO;NF-κB;Phenolics;Red alga
Date: 2010-03-02
Issue Date: 2012-06-18T06:43:48Z
Publisher: Journal of Ethnopharmacology
Abstract: Abstract:
Ethnopharmacological relevance:
Porphyra dentata, a red edible seaweed, has long been used worldwide in folk medicine for the treatment of inflammatory diseases such as hypersensitivity, lymphadenitis, bronchitis.
Aims of study:
To clarify the anti-inflammatory role of Porphyra dentata crude extract and its identified phenolic compounds by investigating their effect on the nitric oxide (NO)/inducible nitric oxide synthase (iNOS) transcription pathway in macrophage RAW 264.7 cells.
Materials and methods:
Porphyra dentata crude extract was prepared with methanol. High performance liquid chromatography (HPLC) hyphenated to electrospray ionization mass spectrometry (ESI-MS) and UV detection were utilized to analyze the extract fingerprints. Nitrite measurement, iNOS promoter activity and nuclear factor-κB (NF-κB) enhancer activity were used to assess the anti-inflammatory effect in lipopolysaccharide (LPS) challenged mouse RAW 264.7 cell line.
Results:
Phenolic compounds (catechol, rutin and hesperidin) were identified in the crude extract of Porphyra dentata. The crude extract and the phenolic compounds inhibited the production of NO in LPS-stimulated RAW 264.7 cells. Catechol was a more potent suppressor of the up-regulation of iNOS promoter and NF-κB enhancer than rutin and yet, hesperidin alone failed to inhibit either activity.
Conclusion:
Our results indicate that catechol and rutin, but not hesperidin, are primary bioactive phenolic compounds in the crude extract to suppress NO production in LPS-stimulated macrophages via NF-κB-dependent iNOS gene transcription. The data also explain the anti-inflammatory use and possible mechanism of Porphyra dentata in iNOS implicated diseases.
Relation: 128(1), pp.123–130
URI: http://ntour.ntou.edu.tw/handle/987654321/32799
Appears in Collections:[食品科學系] 期刊論文

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