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|Title: ||A Novel Optical Scanner Based on Blue-ray DVD Pick-up Head for Analysis of Multifunctional Bio-information|
|Authors: ||Hsing-Cheng Yu;Hong-Chou Lyu;Yuan-Chin Lee;Jau-Jiu Ju;Fu-Jen Kao|
|Contributors: ||NTOU:Department of Systems Engineering and Naval Architecture|
|Issue Date: ||2012-06-15T07:38:04Z
|Publisher: ||Proceedings of the Focus on Microscopy 2011 , Konstanz, Germany|
|Abstract: ||abstract:Fluorescence, laser scanning microscope, blue-ray, DVD, pick-up head. An optical scanner based on a blue-ray DVD pick-up head (BD-PUH) is presented and achieved in Fig. 1 (a). The BD-PUH with a diode laser of 405 nm wavelength can detect various fluorescent dyes and a servo diode laser with 780nm wavelength can control the focusing servo. Additionally, an objective lens on BD-PUH is designed by a non–spherical lens with NA 0.85. The optical resolution of DSM is 0.25 m theoretically. The frame scanner of BD-PUH is designed by a precise biaxial actuator, and it drives the objective lens with 0.5 m resolution for scanning frame images. The servo beam path of BD-PUH offers the auto-focusing function using the focus error signal (FES) detected and estimated from the feedback servo beam upon a photo detector integrated circuit. The emitted fluorescence is detected by a photomultiplier tube (PMT) to obtain high sensitive detection. The fluorescent images can be acquired under photon accumulation of time–correlation single photon counting (TCSPC) . Thus, BD-PUH can optionally work with a TCSPC module and serves as an optical scanner for exciting and detecting fluorescent signals. Furthermore, multifunctional biomedical images can be obtained by interfacing with a TCSPC module . Finally, the dimension of an imaging frame is 256×256 pixels, and the scanning frame rate of DSM can approach to 1 frame/sec. Consequently, the fluorescent intensity images of fibers of lens cleaning paper (Kodak) which labeled with pyranine (in water) are shown in Fig. 1 (b), and (c), respectively. In comparison with BD-PUH, fluorescent paper fiber image has been scanned by Olympus laser scanning confocal microscope (FV-300 and IX-81) which excited by 488nm Argon laser and focused with an objective lens 40X NA 0.85.|
|Appears in Collections:||[系統工程暨造船學系] 演講及研討會|
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