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Application of hCG and LHRH-α to induced breeding of marble goby (Oxyeleotris marmorata)
|Authors: ||Alvin Jap|
|Contributors: ||NTOU:Department of Aquaculture|
Marble goby;Induced breeding;Human Chroionic Gonadotropin;Luteinizing Hormone Releasing Hormone analoque
|Issue Date: ||2012-04-16T01:53:43Z
|Abstract: ||本論文之研究目的為研究雲斑尖塘鱧（Oxyeleotris marmorata）在人工環境下以荷爾蒙注射進行催熟。雲斑尖塘鱧相比於其他淡水魚類具有特別風味，具深亞洲及華人的喜愛。目前雲斑尖塘鱧魚苗的主要來源以野生採捕為主，但是野生魚苗馴餌不易，養殖活存率低，因此產量不高。本研究在人工環境之下使用市售易買之hCG與LHRH-α荷爾蒙進行人工催熟，以應雲斑尖塘鱧產業魚苗之需求。本研究催熟前使用剖腹檢定法判定卵精巢成熟度，以了解種魚成熟之情形，再進行雲斑尖塘鱧之人工催熟。藉由不同濃度之hCG與LHRH-α荷爾蒙及兩種荷爾蒙組合探討最有效及符合經濟效益之濃度，適量之荷爾蒙濃度不影響種魚且可節省成本。另外藉由適量之濃度在人工環境下進行孵化繁殖，最後探討不同鹽度之下對雲斑尖塘鱧初級魚卵孵化及魚苗之活存率。 實驗結果表示，在繁殖季節期進行雲斑尖塘鱧人工催熟可促使未發育之卵精巢發育成熟。雌魚連續兩天注射 hCG 2200 IU/kg BW及hCG 4400 IU/kg BW與雄魚注射連續注射hCG 1100 IU/kg BW三天可促使雲斑尖塘鱧排精排卵。產卵率高達100%，注射第二針後於97.12 ± 0.38 h（Mean ± S.D.）開始產卵， 產卵時間早上06:00到08:00間， 產卵數平均高達為85000顆卵。LHRH-α 濃度（0.11 µg/kg BW ~ 355.55 µg/kg BW）對雲斑尖塘鱧雌魚催熟無法促使排卵，雖然LHRH-α濃度（711.11 µg/kg BW）有促使種魚排精排卵，但魚卵沒有受精，且LHRH-α濃度（1422.22 µg/kg BW）會導致種魚死亡。hCG與LHRH-α荷爾蒙組合使用即使種魚可排精排卵，但是效果不高於單一注射hCG組別，因此本實驗推論雌魚連續兩天注射 hCG 2200 IU/kg BW及hCG 4400 IU/kg BW與雄魚注射hCG 1100 IU/kg BW為雲斑尖塘鱧有效之濃度。 使用不同鹽度0、5、8及10 ppt進行雲斑尖塘鱧孵化繁殖，實驗觀察到0 ppt及5 ppt的孵化環境易使雲斑尖塘鱧魚卵被黴菌感染，0 ppt之下的魚卵在12 h即被黴菌感染而全數死; 孵化在5 ppt之下的大部份魚卵被黴菌感染，倖存的魚苗存活到104 h; 孵化在8 ppt之下僅只有少數卵被黴菌感染，魚苗存活到117 h; 孵化在10 ppt之下的魚卵沒有被黴菌感染，魚卵孵化後成魚苗存活到195 h。|
The purpose of this research is to study induced spawning of marble goby fish (Oxyeleotris marmorata) in remote environment by using hormones. Marble goby flesh has a unique flavor and tender textures, which make marble goby belong to favorites delicacy as a fresh water fish in Asia, mostly in Chinese culture. Fries and juveniles that collected from the river was not easily domesticated to artificial fish pellet, which lead to cannibalism, as this created issues to increase survival rate and marble goby production. For this reason, this research was using commercial hormones such: hCG and LHRH-α, to induce marble goby spawning and finding the most effective concentration to improved marble goby larvae egg and larvae quality. This research was using biopsy surgery method to determine the broodstock fish ovary and testes maturity in pre-hormone injection and post-hormone injection. This experiment was using single hormone hCG and LHRH-α in different concentration independently and mix of both hCG and LHRH-α concentration in order to conferred the most effective and economical concentration to induced spawning of marble goby, and using different salinity 0, 5, 8, 10 ppt to observe egg hatching and larvae survival period afterwards. The experiment resulted in continuous injection of hCG 2200 IU/kg BW on the first day and hCG 4400 IU/kg BW on the second day to female and continuous injection of hCG 1100 IU/kg BW for three days is the most effective and economical concentration to induced spawning of marble goby. Marble goby spawning ratio reached 100%, most pairs spawned averagely at 97.12 ± 0.38 h（Mean ± S.D.）two days after second injection between AM 06:00 to 08:00. Average egg amount 85000. On the other hand, low concentration of 0.11 µg/kg BW ~ 355.55 µg/kg BW LHRH-α were not effective in induced spawning. Even though high concentration of 711.11 µg/kg BW LHRH-α were effective in induced spawning of marble goby, however eggs were not fertilized, injection of 1422.22 µg/kg BW LHRH-α lead broodstock to mortality. Though the result of using of mix hCG and LHRH-α were effective in induced spawning of marble goby but the result is not as effective as hCG group. Using 0 ppt and 5 ppt of salinity to culture marble goby larvae resulted in fertilized eggs were easily infected by fungus (Saprolegnia). 0 ppt of salinity resulted in fertilized eggs died after 12 hours, 5 ppt of salinity resulted in minor amount of fertilized eggs hatch, larvae survived until 104 hours. In comparison of 8 ppt and 10 ppt, major eggs were hatched in 8 ppt larvae survived until 117 hours, eggs were not infected by fungus in 10 ppt, after hatched larvae survive until 195 hours.
|Appears in Collections:||[水產養殖學系] 博碩士論文|
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