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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/30193

Title: 西太平洋海洋性紅藻葉片型龍鬚菜藻種(龍鬚菜科,紅藻門)分子親源關係與分類研究
Molecular phylogeny and taxonomic revision of the marine foliose Gracilaria(Gracilariaceae, Rhodophyta)from the western Pacific Ocean
Authors: Li-Chia Liu
劉麗嘉
Contributors: NTOU:Institute of Marine Biology
國立臺灣海洋大學:海洋生物研究所
Keywords: 龍鬚菜;紅藻;囊果胚胎發育
Date: 2011
Issue Date: 2011-11-25T07:33:46Z
Abstract: 龍鬚菜屬歸類於紅藻植物門、真紅藻綱、龍鬚菜目、龍鬚菜科中,為分布在西太平洋沿岸潮間帶下部及低潮線附近礁岩上常見的海洋紅藻,藻體外觀主要可分為兩型:圓柱狀與葉片狀。葉片型藻種在西太平洋地區藻體常見有鋸齒狀葉片邊緣廣泛被稱為齒葉龍鬚菜、刺邊龍鬚菜或黃氏龍鬚菜;而葉片邊緣為平滑狀的藻種,多被鑑定為葉龍鬚菜或曲葉龍鬚菜。本研究主要結果為以營養生殖結構特徵、囊果胚胎發育之滋養層和管狀營養細胞分佈、雄性生殖窩發育類型的形態比較來重新評估西太平洋葉片型龍鬚菜系統分類以及探討西太平洋地區葉片型龍鬚菜藻種間分子親源關係。 本研究利用 rbcL 基因針對西太平洋地區之葉片型龍鬚菜進行序列分析,分析結果顯示葉片型龍鬚菜之藻種多樣性多於已紀錄之 12 種藻種(楔葉龍鬚菜、黃氏龍鬚菜、曲葉龍鬚菜、斑點龍鬚菜、株龍鬚菜、斯里蘭卡龍鬚菜、刺邊龍鬚菜、長柄龍鬚菜、近岸龍鬚菜、葉龍鬚菜、寬葉龍鬚菜和齒葉龍鬚菜)。分析結果所得的演化分支,可大致以雄性生殖窩類型特徵(Textorii-type,Verrucosa-type,Hydropuntia-type)來做為分群的依據。其中,西太平洋葉片型龍鬚菜共有 15 種,分散於三個演化分支。另一方面,採自台灣及離島沿海的葉片型龍鬚菜顯示藻種間有明顯形態差異,配合分子定序分析結果,顯示台灣產的葉片型龍鬚菜有 7 種,含有 3 種為已紀錄種:刺邊龍鬚菜、黃氏龍鬚菜、斑點龍鬚菜和 4 種世界新種:台灣龍鬚菜、綠島龍鬚菜、柴口龍鬚菜、黑潮龍鬚菜。本研究觀察來自西太平洋葉片型龍鬚菜藻種形態特徵輔以分子分析結果,比較結果得出藻體生長模式、葉片邊緣形狀、藻體短柄高度與直徑、葉片厚度、皮層和髓層層數與細胞大小、囊果胚胎發育的管狀營養細胞分佈、滋養層厚度與滋養層細胞形狀等等形質,大都可做為辨識藻種的形態特徵,可以應用於葉片型龍鬚菜藻種的分類探討。
Foliose species of Gracilaria (Gracilariaceae, Rhodophyta) are common red algae found on the rocky shore or coral reefs in intertidal to subtidal zone of western Pacific Ocean. Previous studies have shown that foliose species have two types of thallus morphology: blades with spiny margins (e.g. G. vieillardii, G. spinulosa, G. huangii) and blades with smooth margins (e.g. G. textorii, G. incurvata). In this study, the phylogenetic relationships among the foliose Gracilaria species from Western Pacific Ocean are inferred based on rbcL sequence analyses and the morphological characters such as tubular-nutritive cell, floor of cystocarp and spermatangial conceptacle development are used for delineating the species boundaries. Our rbcL sequence analysis showed that foliose Gracilaria analyzed were grouped into three evolutionary lineages (= clades): first clade containing the species possessing Textorii-type of spermatangial sori, second clade containing the species possessing Verrucosa-type of spermatangial sori, and the third clade containing the species possessing Hydropuntia-type of spermatangial sori. The species diversity of foliose Gracilaria in the western Pacific Ocean is much higher than previously thought. The numbers of the foliose species is increased to twelve species (G. cuneifolia, G. huangii, G. incurvata, G. puncktata, G. pygmaea, G. srilankia, G. spinulosa, G. stipitata, G. sublittoralis, G. textorii, G. truncata, G. vieillardii). The species were previously recorded as G. “vieillardii” or G. “textorii” from Taiwan and the neighboring islands are actually a new species (named as G. taiwanensis Liu, Lin et Payri herein) and G. punctata, respectively. In total, four new foliose species (G. taiwanensis sp. nov., G. ludouensis sp. nov., G. chaikouensis sp. nov., G. kurushioensis sp. nov.) are newly described in this study. Based on the supporting evidence resulting from rbcL sequence analysis, the morphological characters, which can be used for separating the foliose Gracilaria found in the western Pacific Ocean are growth pattern of thalli, morphology of blade margins, length and branching pattern of stipes, blade thickness and layer numbers of cortex and medulla, distribution of tubular-nutritive cells in cystocarp cavities, cell morphology and thickness of cystocarp floor etc.
URI: http://ethesys.lib.ntou.edu.tw/cdrfb3/record/#G0M98340001
http://ntour.ntou.edu.tw/handle/987654321/30193
Appears in Collections:[海洋生物研究所] 博碩士論文

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