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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/29516

Title: White spot syndrome virus envelope protein VP53A interacts with Penaeus monodon chitin-binding protein (PmCBP)
Authors: Li-Li Chen;Li-Chen Lu;Wan-Jung Wu;Chu-Fang Lo;Wei-Pang Huang
Contributors: NTOU:Institute of Marine Biology
Keywords: Penaeus monodon;White spot syndrome virus;VP53A;Envelope protein;Chitin-binding protein
Date: 2007
Issue Date: 2011-10-21T03:06:48Z
Publisher: Dis Aquat Org
Abstract: Abstract: White spot syndrome virus (WSSV) is the causative agent of a severe disease of cultivated shrimp. Using purified WSSV virions, VP53A encoded by open reading frame wssv067 was identified as a structural protein by SDS-PAGE and proteomics. Immunoelectron microscopy with a gold-labeled secondary antibody revealed that VP53A was distributed on the viral envelope. In order to further explore the link between WSSV067 and host proteins, we performed a yeast 2-hybrid screening of a Penaeus monodon cDNA library, using WSSV067C as bait. One of the molecules that specifically interacted with WSSV067C was the P. monodon chitin-binding protein (PmCBP). An in vitro binding assay showed that c-myc-WSSV067C was capable of co-precipitating HA-PmCBP-C. Furthermore, PmCBP was expressed in almost all organs but appeared to be up-regulated at the late stage of WSSV infection.
Relation: 74, pp.171-178
URI: http://ntour.ntou.edu.tw/handle/987654321/29516
Appears in Collections:[海洋生物研究所] 期刊論文

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