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题名: The Identification of Three Novel Genes Involved in the Rapid-Growth Regulation in a Marine Diatom, Skeletonema costatum
作者: Chih-Ching Chung;Sheng-Ping L. Hwang;Jeng Chang
贡献者: NTOU:Institute of Marine Biology
國立臺灣海洋大學:海洋生物研究所
关键词: Subtraction PCR;Indicator;Growth;Diatom
日期: 2009-06
上传时间: 2011-10-21T03:06:35Z
出版者: Marine Biotechnology
摘要: Abstract:In order to understand the molecular mechanism of growth regulation in phytoplankton and to develop novel growth-status indicators, a subtraction cDNA library was constructed by using the mRNA extracted from Skeletonema costatum in the rapid-growth stage (RG stage), and three RG-stage-related cDNA fragments, RG#14, RG#25, and RG#42, were obtained. According to the results of sequence analysis, RG#42 belonged to the MCM2-7 protein family, and the other two fragments, RG#14 and RG#25, were novel molecules. Under continuous illumination, these RG-stage-related mRNA expression levels increased from 100- (RG#14 and RG#42) to 1,000-fold (RG#25) with increasing growth rate. Furthermore, under a diel rhythm of light (light–dark = 12:12 h), the daily mean mRNA abundances of RG#14 and RG#25 in the exponential phase also differed from those in the late-stationary phase. However, such differences between these growth phases were not observed in the mRNA levels of RG#42 and PCNA. This study not only provided a new way to investigate the regulatory mechanisms of cell growth but also offered a possibility of employing these gene fragments as indicators to monitor the growth status of phytoplankton in the marine environment.
關聯: 11(3), pp.356-367
URI: http://ntour.ntou.edu.tw/handle/987654321/29498
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