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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/29425

Title: Phthalate degradation by mangrove soil fungi
Authors: Luo Z. H.;Pang K. L.;Gu J. D.;Vrijmoed L. L. P.
Contributors: NTOU:Institute of Marine Biology
Keywords: Degradation;fungi;mangrove sediment;phthalate esters
Date: 2007
Issue Date: 2011-10-21T03:05:53Z
Publisher: 5th International Conference on Marine Pollution and Ecotoxicology
Abstract: Abstract:Phthalate esters are synthetic compounds used as plasticizers to improve mechanical properties of the plastic resin, particularly flexibility. In recent years, they have attracted increasing attention owing to their widespread use, ubiquity in the environment, and endocrine-disrupting activity(ability to human?). Degradation mechanisms and pathways of phthalate esters have been consolidated in bacteria while those in fungi have not been shown. This study reports the enrichment and isolation of phthalate-degrading fungi from mangrove sediment samples collected at various locations in Futian Nature Reserve in Shenzhen, China. Initial fungal cultures were established by inoculating 5 g of sediment into mineral salts liquid medium supplemented with 100 mg/l of three isomers of dimethyl phthalate ester (DMPE), including dimethyl phthalate (DMP), dimethyl isophthalate (DMI), and dimethyl terephthalate (DMT), as the sole source of carbon and energy. These enrichment cultures were transferred to fresh enrichment liquid medium every week for a total of five weeks. Medium from the final enrichment culture will be plated out on agar plates for the isolation of pure cultures of DMPE-degrading fungi. Results on the depletion assay to determine the rate of DMPE utilization and the intermediate products of DMPE degradation by these fungi will be presented.
URI: http://ntour.ntou.edu.tw/handle/987654321/29425
Appears in Collections:[海洋生物研究所] 演講及研討會

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