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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/29415

Title: Penaeus monodon chitin-binding protein (PmCBP) is involved in white spot syndrome virus (WSSV) infection
Authors: Kuan-Yu Chen;Tai-Ching Hsu;Po-Yu Huang;Shih-Ting Kang;Chu-Fang Lo;Wei-Pang Huang;Li-Li Chen
Contributors: NTOU:Institute of Marine Biology
Keywords: White spot syndrome virus (WSSV);Envelope protein;VP53A;Penaeus monodon chitin-binding protein (PmCBP);In vivo neutralization
Date: 2009-09
Issue Date: 2011-10-21T03:05:48Z
Publisher: Fish & Shellfish Immunology
Abstract: Abstract:White spot syndrome virus (WSSV) can cause the most serious viral disease of shrimp and has a wide host range among crustaceans. Although researches show a lot about its genome and structure, information concerning the mechanism of how WSSV infects' cells is lacking. In this study, some experiments were applied to confirm the biological meaning of the protein–protein interaction between WSSV envelope protein, VP53A, and Penaeus monodon chitin-binding protein (PmCBP). Immunofluorescent study indicated that PmCBP is located on the cell surface of host cells. PmCBP amounts of about 34 kDa can be detected in both P. monodon and Litopenaeus vannamei tissues by Western blotting. In the in vivo neutralization experiment, both rVP53A and rPmCBP that were produced by Esherichia coli can promote resp. a 40% and 20% survival rate of the shrimp which were challenged by WSSV. Furthermore, a yeast-two-hybrid result revealed that PmCBP could interact with at least 11 WSSV envelope proteins. Those findings suggest that PmCBP may be involved in WSSV infection.
Relation: 27(3), pp.460–465
URI: http://ntour.ntou.edu.tw/handle/987654321/29415
Appears in Collections:[海洋生物研究所] 期刊論文

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