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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/29401

Title: Nitric Oxide as a Signaling Factor To Upregulate the Death-Specific Protein in a Marine Diatom, Skeletonema costatum, during Blockage of Electron Flow in Photosynthesis
Authors: Chih-Ching Chung;Sheng-Ping L. Hwang;Jeng Chang
Contributors: NTOU:Institute of Marine Biology
國立臺灣海洋大學:海洋生物研究所
Date: 2008-11
Issue Date: 2011-10-21T03:05:42Z
Publisher: Applied and Environmental Microbiology
Abstract: Abstract:To determine the physiological functions of a novel death-specific protein gene, Skeletonema costatum DSP-1 (ScDSP-1) in a marine diatom, Skeletonema costatum, the mRNA abundance of ScDSP-1 was measured in cultures subjected to light manipulation and treatments with various chemicals. When cells were transferred to a dim light intensity of 15 μmol m−2 s−1, ScDSP-1 mRNA levels showed a transient increase of 1 to 17.2 μmol (mol 18S rRNA)−1 in 60 h. Furthermore, treatments with the photoinhibitors 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB) resulted in high ScDSP-1 mRNA levels, which reached 943 and 72 μmol (mol 18S rRNA)−1, respectively. Treatment with the nitric oxide (NO) donor diethylamine nitric oxide also induced ScDSP-1 expression, and this inducible expression was inhibited by the NO scavenger hemoglobin. Additionally, the expression of ScDSP-1 mRNA elicited by DCMU and DBMIB was efficiently reduced when cultures were pretreated with the cell-penetrating NO scavenger 2-(4-carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide. In contrast, treatment with another photoinhibitor, paraquat, had no effect on ScDSP-1 expression. Our results indicated that NO is the crucial secondary messenger which signals the expression of ScDSP-1 when electron flow between photosystem II and photosystem I is blocked in S. costatum cells. In addition, the discovery of a similar gene, ScDSP-2, is briefly described.
Relation: 74(21), pp.6521-6527
URI: http://ntour.ntou.edu.tw/handle/987654321/29401
Appears in Collections:[海洋生物研究所] 期刊論文

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