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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/29396

Title: New primers for detection of Smittium spp. (Trichomycetes, Zygomycota) in insect hosts
Authors: Rizzo A. M.;Pang K. L.
Contributors: NTOU:Institute of Marine Biology
Keywords: Aedes;Culex;fungi;Harpellales;rRNA
Date: 2005
Issue Date: 2011-10-21T03:05:41Z
Publisher: Fungal Diversity
Abstract: Abstract:The distinctive thalli of trichomycetes can only be isolated from the larval stage of the host. There is a general lack of knowledge on the presence of trichomycetes in other developmental stages of their host. Two new primers, TR3F and TR4R, were designed based on SSU ribosomal DNA sequences from two Harpella species, one Harpellomyces species, four Smittium species and Aedes aegypti (host). These primers amplified DNA from species of the Harpellales but discriminated against other groups of organisms including other fungal phyla. The presence of Smittium species was detected in laboratory-infected pupae and adult flies of Aedes aegypti and in field-collected larvae of Culex pipiens by PCR using the new primers.
Relation: 19, pp.129-136
URI: http://ntour.ntou.edu.tw/handle/987654321/29396
Appears in Collections:[海洋生物研究所] 期刊論文

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