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|Title: ||Molecular perspectives on assessing fungal diversity from marine substrates|
|Authors: ||Pang K. L.;Mitchell J. I.|
|Contributors: ||NTOU:Institute of Marine Biology|
|Keywords: ||Ecology;marine fungi;PCR primers;rRN|
|Issue Date: ||2011-10-21T03:05:33Z
|Publisher: ||Botanica Marina|
|Abstract: ||Abstract:A range of molecular techniques is available to assess fungal diversity in natural environments. These include denaturing gradient gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE), single-strand conformation polymorphism (SSCP), terminal restriction fragment length polymorphism (T-RFLP), amplified rDNA restriction analysis (ARDRA), amplified ribosomal intergenic spacer analysis (ARISA) and cloning. The techniques, PCR primers for environmental work and their potential limitations are discussed in this review, together with applications for describing the diversity of filamentous marine fungi associated with their substrata. It is unlikely that a single technique can be used to assess all aspects of diversity, however the limited number of studies with a molecular approach has demonstrated that marine substrata contain a greater fungal diversity than previously recognised. These studies have also shown that it is important to combine mycological and molecular approaches to accurately assess diversity.|
|Relation: ||48, pp.332-347|
|Appears in Collections:||[海洋生物研究所] 期刊論文|
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