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|Title: ||Molecular detection of bacteria in the seventeenth century Swedish warship Vasa|
|Authors: ||Pang K. L.;Eaton R. A.;Mitchell J. I.|
|Contributors: ||NTOU:Institute of Marine Biology|
|Keywords: ||Archaea;bacteria;molecular diversity;wooden ship|
|Issue Date: ||2011-10-21T03:05:31Z
|Publisher: ||Proceedings of the 9th ICOM Group on Wet Organic Archaeological Materials Conference|
|Abstract: ||Abstract:A high level of acidity has been recorded in the conserved timbers of the hull and objects of the seventeenth century Swedish warship Vasa. This may have resulted from the oxidation of accumulated hydrogen sulphide to sulphuric acid and the formation of acid may be due to the activity of microorganisms in the ship's timbers. This study was initiated to determine if (a) bacteria and Archaea are present in the ship's timbers and (b) to assess whether these organisms are active. Wood cores taken from different parts of the ship and wooden objects of oak and pine were extracted for total DNA and RNA to determine the presence of bacterial and archaeal communities (DNA) and to assess their activity (RNA). Total DNA was isolated from the samples and partial 16S rDNA was amplified using primers specific to bacteria which indicates their presence in the wood. RNA was also successfully extracted with the partial 16S rRNA amplified using the same primers by one-step reverse transcription-polymerase chain reaction (RT-PCR). This result suggests that active bacterial groups, which might be involved in the production of sulphuric acid in the wood of the Vasa, are present. The identity of these active bacterial groups is essential to find a cure for the sulphur problem in the Vasa timbers.|
|Appears in Collections:||[海洋生物研究所] 演講及研討會|
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