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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/29364

Title: Immunohistochemical identification of hyperglycemic hormone- and molt-inhibiting hormone-producing cells in the eyestalk of the kuruma prawn, Penaeus japonicus
Authors: Tung-Wei Shih;Yuzuru Suzuki;Hiromichi Nagasawa;Katsumi Aida
Contributors: NTOU:Institute of Marine Biology
國立臺灣海洋大學:海洋生物研究所
Date: 1998-06-01
Issue Date: 2011-10-21T03:05:29Z
Publisher: Zoological Society of Japan
Abstract: Abstract:This study deals with the localization of crustacean hyperglycemic hormone (CHH, Pej-SGPIII) and molt-inhibiting hormone (MIH, Pej-SGP-IV) in the eyestalk of the kuruma prawn Penaeus japonicus using immunohistochemistry. High-titer and highly specific antisera were raised in rabbits against synthetic Pej-SGP-III C-terminal peptide (Glu-Glu-His-Met-Ala-Ala-Met-Gln-Thr-Val-NH2) and Pej-SGP-IV C-terminal peptide (Val-Trp-Ile-Ser-Ile-Leu-Asn-Ala-Gly-Gln-OH), both of which were conjugated with bovine serum albumin by a cross linker. Eyestalks were removed from mature male prawns at the intermolt stage of the molting cycle and fixed in Bouin's solution. Serial sections stained immunohistochemically showed that neurosecretory cells of Pej-SGP-III and Pej-SGP-IV were located in the same cluster of the medulla terminalis ganglionic X-organ (MTGX), and that three kinds of neurosecretory cells, which were stained with anti-PejSGP-III antiserum and/or anti-Pej-SGP-IV antiserum were present. The number of neurosecretory cells which stained with both antisera was much fewer than that of neurosecretory cells which stained with one of the antisera only. The axon and axon terminals in the sinus gland were also stained and the staining density of the sinus gland was always deeper than that of the neurosecretory cells.
Relation: 15(3), pp.389-397
URI: http://ntour.ntou.edu.tw/handle/987654321/29364
Appears in Collections:[海洋生物研究所] 期刊論文

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