English  |  正體中文  |  简体中文  |  Items with full text/Total items : 26994/38795
Visitors : 2390941      Online Users : 128
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/29325

Title: Hemolymph vitellogenin levels during ovarian development in the kuruma prawn Penaeus japonicus
Authors: Safiah Jasmani;Ichiro Kawazoe;Tung-Wei Shih;Yuzuru Suzuki;Katsumi Aida
Contributors: NTOU:Institute of Marine Biology
國立臺灣海洋大學:海洋生物研究所
Keywords: enzyme immunoassay;kuruma prawn;vitellogenesis;vitellogenin
Date: 2000-06
Issue Date: 2011-10-21T03:05:18Z
Publisher: Fisheries Science
Abstract: Abstract:
SUMMARY:An enzyme immunoassay (EIA) system for kuruma prawn Penaeus japonicus vitellogenin (Vg) was developed and validated. In this assay, 96-well microtiter plates were coated with free vitellin (Vt). Assay conditions provided a detectable Vg range of 1.56–100 ng/well. The dose–response curve for serially diluted vitellogenic female hemolymph was parallel to the Vg standard curve. Male hemolymph produced absorbance values almost equal to background values, confirming the specificity of the assay. The intra- and interassay coefficient of variations were 6.0% and 10.5%, respectively. Using this assay, it was determined that very low levels of hemolymph Vg were detectable in prawns with oocytes at the previtellogenic stage. The Vg levels in the hemolymph rapidly increased at the endogenous vitellogenic stage and peaked at the early exogenous vitellogenic stage, but decreased at the late exogenous vitellogenic stage. Changes in hemolymph Vg levels preceded observed changes in gonadosomatic indices.
Relation: 66(3), pp.535–539
URI: http://ntour.ntou.edu.tw/handle/987654321/29325
Appears in Collections:[海洋生物研究所] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML276View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback