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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/28799

Title: Improvement of frequency sensitivity of series photodetector frequency circuit system and its application for Cy5 fluorescence detection
Authors: Yuh Ming Hsu;Chung Cheng Chang
Contributors: NTOU:Department of Electrical Engineering
國立臺灣海洋大學:電機工程學系
Keywords: Series photodetector frequency circuit system;Photodetector;DNA probe;Fluorescence dye concentration;Cy5
Date: 2011-10
Issue Date: 2011-10-21T02:40:42Z
Publisher: Optik
Abstract: Abstract:This study elucidated the frequency characteristics of series photodetector frequency circuit system for detection of DNA probe ET996 marked with fluorescence dye Cy5. We developed 48 MHz series photodetector frequency circuit system with good sensitivity for fluorescence detection. In accordance with the theory of series photodetector frequency circuit system, the frequency sensitivity can be improved by adjusting circuit parameters such as A (tan θ), Cq, C0, and Cp. In this research of A adjustment, the capacitance parameter Cm of 48 MHz series photodetector frequency circuit system was adjusted to improve the frequency sensitivity for detection of fluorescence dye concentration; moreover, the bias of photodetector was also adjusted to improve the frequency sensitivity. In the optimal conditions of capacitance match and photodetector bias, the detection limit of ET996-Cy5 fluorescence dye concentration 2 pmol/L can be measured by 48 MHz sensor system. The results of fluorescence experiment also demonstrated that the frequency shift of 48 MHz sensor system was linearly related to the logarithm of fluorescence dye concentration from 200 nmol/L to 2 pmol/L. The frequency method can be applied simply and the detection limit of ET996-Cy5 fluorescence dye concentration was lower than the conventional fluorescence technique by 2 orders.
Relation: 122(19), pp.1747-1752
URI: http://ntour.ntou.edu.tw/handle/987654321/28799
Appears in Collections:[電機工程學系] 期刊論文

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