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|Title: ||Inhibition of the Interactions between Eosinophil Cationic Protein and Airway Epithelial Cells by Traditional Chinese Herbs|
|Authors: ||Hao-Teng Chang;Louis J. Tseng;Ta-Jen Hung;Blacky T. Kao;Wei-Yong Lin;Tan-chi Fan;Margaret Dah-Tsyr Chang;Tun-Wen Pai|
|Contributors: ||NTOU:Department of Computer Science and Engineering|
|Issue Date: ||2011-10-21T02:35:08Z
|Publisher: ||BMC Systems Biology|
Background:The eosinophil cationic protein (ECP) is cytotoxic to bacteria, viruses, parasites and mammalian cells. Cells are damaged via processes of pore formation, permeability alteration and membrane leaking. Some clinical studies indicate that ECP gathers in the bronchial tract of asthma sufferers, damages bronchial and airway epithelial cells, and leads to in breathing tract inflammation; therefore, prevention of the cytotoxicity caused by ECP may serve as an approach to treat airway inflammation. To achieve the purpose, reduction of the ECP-cell interactions is rational. In this work, the Chinese herbal combinative network was generated to predict and identify the functional herbs from the pools of prescriptions. It was useful to select the node herbs and to emonstrate the relative binding ability between ECP and Beas-2B cells with or withour herb treatments. Results : Eighty three Chinese herbs and prescriptions were tested and five effective herbs and six prescription candidates were selected. On the basis of effective single-herbal drugs and prescriptions, a combinative network was generated. We found that a single herb, Gan-cao, served as a node connecting five prescriptions. In addition, Sheng-di-huang, Dang-guei and Mu-tong also appeared in five, four and three kinds of prescriptions, respectively. The extracts of these three herbs indeed effectively inhibited the interactions between ECP and Beas-2B cells. According to the Chinese herbal combinative network, eight of the effective herbal extracts showed inhibitory effects for ECP internalizing into Beas-2B cells. The major components of Gang-cao and Sheng-di-huang, glycyrrhizic acid and verbascose, respectively, reduced the binding affinity between ECP and cells effectively. Conclusions : Since these Chinese herbs reduced the binding affinity between ECP and cells and inhibited subsequent ECP entrance into cells, they were potential for mitigating the airway inflammation symptoms.
|Appears in Collections:||[資訊工程學系] 期刊論文|
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