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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27811

Title: Development and evaluation of a sensitive enzyme-linked oligosorbent assay for detection of polymerase chain reaction amplified hepatitis C virus of genotypes 1 to 6
Authors: Rong-Yuan Huang;Hao-Teng Chang;Chung-Yu Lan;Tun-Wen Pai;Chao-Nan Wu;Chung-Mei Ling;Margaret Dah-Tsyr Chang
Contributors: NTOU:Department of Computer Science and Engineering
Keywords: Hepatitis C virus;PCR-ELOSA;Genotyping;HCV
Date: 2008
Issue Date: 2011-10-21T02:34:06Z
Publisher: Journal of Virological Methods
Abstract: Abstract:A high-throughput polymerase chain reaction (PCR)-based enzyme-linked oligonucleotide-sorbent assay ELOSA)was developed for use in the diagnostic testing of serum from patients who may be infected with different hepatitis C virus (HCV) genotypes. Twelve genotype-specific 5 -aminated DNA-coated probes were designed based on the variable 5 -untranslated region sequences of the HCV genotypes 1–6. Using 100 clinical serum samples, the performance of the PCR-ELOSAmethodwascompared with Roche’s COBAS Amplicor HCV Monitor V2.0 assay and the VERSANT HCV genotype assay (LiPA), and the overall agreement was 99% at the level of HCV genotypes with a detection range of 2.0×102 to 1.0×107 IU/ml for PCR-ELOSA. The PCR-ELOSA was more comprehensive as demonstrated by the fact that ?20% of the samples with different subtypes could be discriminated by this method but not by LiPA. In addition, the PCR-ELOSA system showed high accuracy (CV?6.36%) and even higher reproducibility (CV?5.55%). Thus, this novel PCR-ELOSA system provides a sensitive and versatile alternative to current HCV detection assays.
Relation: 151(2), pp.211-216
URI: http://ntour.ntou.edu.tw/handle/987654321/27811
Appears in Collections:[資訊工程學系] 期刊論文

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