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题名: Aligned multiple-walled carbon nanotubes as biosensors by colorimetric and amperometric techniques
作者: JING-RU WU;EDWIN JAHNGEN;KUANG-PANG LI;WAN-SUN TSE;PING CHIANG;HAI-PANG CHIANG
贡献者: NTOU:Institute of Optoelectronic Sciences
國立臺灣海洋大學:光電科學研究所
关键词: Carbon nanotubes;biosensors;horseradish peroxidase;phenols
日期: 2005-04
上传时间: 2011-10-21T02:31:45Z
出版者: International Journal of Nanoscience
摘要: Abstract:Vertically oriented multiple-walled carbon nanotubes (MWCNTs) aligned on a silicon substrate were covalently bonded with horseradish peroxidase (HRP) and used as biosensors or electrodes. The modified platform was investigated with Scanning Electron Microscopy (SEM), UV-Vis spectrophotometer and Cyclic Voltammetry (CV). The enzyme catalyzed reaction and the subsequent reduction of the enzymatic products by the platform were carefully investigated. The assay was done by measuring the absorbance of the dye formed from the reaction product of the substrates (phenol) and 4-aminoantipyrine. From the linear response, phenol can be quantitized in the range from 47 ppm to 750 ppm with a detection limit of 19 ppm (based on S/N = 3). The resulting modified aligned MWCNTs still exhibited enzymatic activities after storage of 13 days. The activity of the attached HRP was also demonstrated electrochemically.
關聯: 4(2), pp.245-252
URI: http://ntour.ntou.edu.tw/handle/987654321/27430
显示于类别:[光電科學研究所] 期刊論文

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