National Taiwan Ocean University Institutional Repository:Item 987654321/27430
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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27430

Title: Aligned multiple-walled carbon nanotubes as biosensors by colorimetric and amperometric techniques
Authors: JING-RU WU;EDWIN JAHNGEN;KUANG-PANG LI;WAN-SUN TSE;PING CHIANG;HAI-PANG CHIANG
Contributors: NTOU:Institute of Optoelectronic Sciences
國立臺灣海洋大學:光電科學研究所
Keywords: Carbon nanotubes;biosensors;horseradish peroxidase;phenols
Date: 2005-04
Issue Date: 2011-10-21T02:31:45Z
Publisher: International Journal of Nanoscience
Abstract: Abstract:Vertically oriented multiple-walled carbon nanotubes (MWCNTs) aligned on a silicon substrate were covalently bonded with horseradish peroxidase (HRP) and used as biosensors or electrodes. The modified platform was investigated with Scanning Electron Microscopy (SEM), UV-Vis spectrophotometer and Cyclic Voltammetry (CV). The enzyme catalyzed reaction and the subsequent reduction of the enzymatic products by the platform were carefully investigated. The assay was done by measuring the absorbance of the dye formed from the reaction product of the substrates (phenol) and 4-aminoantipyrine. From the linear response, phenol can be quantitized in the range from 47 ppm to 750 ppm with a detection limit of 19 ppm (based on S/N = 3). The resulting modified aligned MWCNTs still exhibited enzymatic activities after storage of 13 days. The activity of the attached HRP was also demonstrated electrochemically.
Relation: 4(2), pp.245-252
URI: http://ntour.ntou.edu.tw/handle/987654321/27430
Appears in Collections:[Institute of Optoelectronic Sciences] Periodical Articles

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