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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27417

Title: Chemo-enzymatic Synthesis of Trimeric Sialyl Lewisx Pentadecasaccharide
Authors: Yeuk Chuen Liu;Hong Li;Albin Otter;Vivekanand P Kamath;Markus B Streiff;Monica M Palcic
Contributors: NTOU:Department of Food Science
國立臺灣海洋大學:食品科學系
Keywords: Chemo-enzymatic Synthesis;Sialyl Lewisx;oligosaccharide
Date: 2002-06
Issue Date: 2011-10-21T02:25:19Z
Publisher: Canadian Journal of Chemistry
Abstract: Abstract:The enzymatic synthesis of trimeric sialyl Lewisx pentadecasaccharide (6), a 15-mer, from a trimannoside precursor required six different glycosyltransferase enzymes and four nucleotide donor sugars. Three N-acetylglucosaminyl residues were transferred from UDP-N-acetylglucosamine to a trimannoside by N-acetylglucosaminyltransferases I, II, and V, respectively. Galactosylation using β(14) galactosyltransferase and UDP-galactose gave three N-acetyl lactosamine units in nonasaccharide 4. Sialylation of 4 with α(23) sialyltransferase and CMP-N-acetylneuraminic acid was followed by fucosylation with α(13) fucosyltransferase and GDP-fucose giving the 15-mer 6 in mg quantities. Compound 4 was also converted to a trimeric Lewisx dodecasaccharide 12-mer with α(13) fucosyltransferase and GDP-fucose and to a trimeric α-2,6-sialyl N-acetyllactosamine dodecasaccharide 12-mer with α(26) sialyltransferase and CMP-N-acetylneuraminic acid. Key words: glycosyltransferases, pentadecasaccharide, sialyl Lewisx.
Relation: 80(6), pp.540-545
URI: http://ntour.ntou.edu.tw/handle/987654321/27417
Appears in Collections:[食品科學系] 期刊論文

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