English  |  正體中文  |  简体中文  |  Items with full text/Total items : 28603/40634
Visitors : 4332379      Online Users : 270
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27411

Title: Apoptosis-inducing active components from Corbicula fluminea through activation of caspase-2 and production of reactive oxygen species in human leukemia HL-60 cells
Authors: Ying-Tang Huang;Yi-Hsuan Huang;Tzhy-Chyuan Hour;Bonnie Sun Pan;Yeuk-Chuen Liu;Min-Hsiung Pan
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Corbiculafluminea Muller;Reactiveoxygenspecies;Apoptosis;Cytochrome c;Caspase-9;Caspase-3;Poly(ADP-ribose) polymerase;DNA fragmentation factor;Caspase-activated deoxyribonuclease;Bax;Bad;Bcl-2;Bcl-XL;FME, Freshwater clam extracted with methanol then partitioned with ethyl acetate
Date: 2006-08
Issue Date: 2011-10-21T02:25:16Z
Publisher: Food and Chemical Toxicology
Abstract: Abstract:The anti-cancer effects and possible mechanisms of the freshwater clam (Corbicula fluminea Muller) and its active compounds (FME) on cell viability in human leukemia HL-60 cells were investigated. This study demonstrated that FME was able to inhibit cell proliferation in a concentration- and time-dependent manner. Treatment with FME caused induction of caspase-2, caspase-3, caspase-6, caspase-8, and caspase-9 activity in a time-dependent manner, but not affect caspase-1 activity; it induced the proteolysis of DNA fragmentation factor (DFF-45) and poly(ADP-ribose) polymerase (PARP). Induction of cell death by FME was completely prevented by a pan-caspase inhibitor, Z-Val-Ala-Asp-fluoromethyl ketone (Z-VAD-FMK) and a caspase-2 inhibitor, Z-Val-Asp-Val-Ala-Asp-FMK (Z-VDVAD-FMK). Furthermore, treatment with FME caused a rapid loss of mitochondrial transmembrane potential, stimulation of generation of reactive oxygen species (ROS), release of mitochondrial cytochrome c into cytosol, and GSH depletion. Anti-oxidants such as N-acetylcysteine, catalase, superoxide dismutase, allopurinol, and pyrrolidine dithiocarbamate, but not diphenylene iodonium, significantly inhibited FME-induced cell death. In addition, the results showed that FME-induced apoptosis was accompanied by up-regulation of Bax and Bad, and down-regulation of Bcl-2 and Bcl-XL. Taken together, induction of apoptosis on HL-60 cells by FME was mainly associated with ROS production, GSH depletion, mitochondrial dysfunction, and caspase activation.
Relation: 44(8), pp.1261–1272
URI: http://ntour.ntou.edu.tw/handle/987654321/27411
Appears in Collections:[食品科學系] 期刊論文

Files in This Item:

File Description SizeFormat

All items in NTOUR are protected by copyright, with all rights reserved.


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback