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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27401

Title: Molecular Characterization of Mungbean (Vigna radiata L.) Starch Branching Enzyme I
Authors: Jia-Wei Chang;Sing-Chung Li;Yun-Chi Shih;Reuben Wang;Pei-Shan Chung;Yuan-Tih Ko
Contributors: NTOU:Department of Food Science
國立臺灣海洋大學:食品科學系
Keywords: Mungbean;starch branching enzyme;gene expression;recombinant enzyme
Date: 2010
Issue Date: 2011-10-21T02:25:09Z
Publisher: J Agric Food Chem
Abstract: Abstract:Mungbean (Vigna radiata L. cv. Tainan no. 5) starch branching enzyme I (SBE, EC 2.4.1.18) cDNA, VrsbeI, was cloned, and its expression was characterized. Conserved regions of the family B SBE were used to amplify a full length cDNA of 2208 bp. Phylogeny was analyzed, and the partial 3D structure and functional features were predicted. Catalytic residues were identified in the (α/β)8-fold, and a unique loop from F365 to F376 between β3/α3 was located. Gene expression of VrsbeI in seeds during growth showed that the transcript appeared from week 1 and increased substantially at week 3−4. It was cloned into the pET30 vector and expressed in E. coli BL21(DE3) pLysS cells as a soluble recombinant protein. The affinity-purified recombinant VrSBEI exhibited a specific activity of 314.6 U/mg as an active enzyme with 114-fold activity enrichment from the crude extract.
Relation: 58(19), pp.10437–10444
URI: http://ntour.ntou.edu.tw/handle/987654321/27401
Appears in Collections:[食品科學系] 期刊論文

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