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Title: Tetrodotoxin in several species of xanthid crabs in southern Taiwan
Authors: Yung-Hsiang Tsai;Ping-Ho Ho;Chiu-Chi Hwang;Pai-An Hwang;Chao-An Cheng;Deng-Fwu Hwang
Contributors: NTOU:Department of Food Science
Keywords: Xanthid crab;Tetrodotoxin;Toxicity;LC-MS;GC-MS
Date: 2006-03
Issue Date: 2011-10-21T02:24:22Z
Publisher: Food Chemistry
Abstract: Abstract:Tetrodotoxin was detected by tetrodotoxin (TTX) bioassay in 28 specimens of six speciesofxanthidcrabs collected from Tunkang, southernTaiwan, in May 2004. The average toxicity ofcrab specimens was 347 ± 276 (means ± SD) mouse units (MU) for Demania cultripes, 59 ± 17 MU for Demania toxica, 324 ± 114 MU for Demania reynaudi, 214 ± 65 MU for Lophozozymus incisus, 184 ± 85 MU for Lophozozymus pictor and 611 ± 471 MU for Atergatopsis germaini. Each toxin of the six speciesofcrabs was extracted with acidic methanol, cleansed using a C18 solid-phase extraction (SPE) column, filtered through a microcentrifuge filter and determined by LC-MS and GC-MS. The recovery of TTX was above 82.9% and the detection limit was 0.005 μg with LC-MS analysis. A linear equation was obtained between 0.03 and 3.0 μg of TTX. Analyses by LC-MS and GC-MS showed that these crabspecies all contained TTX. The paralytic shellfish poisons were not found. Among samples, D. cultripes, D. toxica and L. incisus are the first to be recorded as toxic in Taiwan.
Relation: 95(2), pp.205-212
Appears in Collections:[Department of Environmental Biology and Fisheries Science] Periodical Articles
[Department of Food Science] Periodical Articles

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