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题名: The receptor of an oyster juice-borne coliphage OJ367 in the outer membrane of Salmonella derby
作者: Yuan-Tih Ko
贡献者: NTOU:Department of Food Science
國立臺灣海洋大學:食品科學系
日期: 2005-12
上传时间: 2011-10-21T02:24:20Z
出版者: Journal of Microbiology, Immunology and Infection
摘要: Abstract:The objective of this study was to identify the receptor of OJ367, an oyster juice-borne bacteriophage, in Salmonella derby ATCC 6960. The crude receptor outer membrane (OM) fraction was prepared and examined from the total cell envelope (TCE) by differential extraction with N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid (HEPES)-MgCl2 and then with Triton-HEPES-ethylenediamine tetra-acetic acid buffers. The OM proteins (Omps) were isolated by diethylaminoethyl column chromatography to screen for receptor activity. A 45-kDa protein belonging to a minor Omp species, with phage neutralization ability, was eluted in a homogeneous form. It was a non-peptidoglycan-associated protein which was digestible by trypsin. Lipopolysaccharide had no influence on its receptor activity when coexistent in the diethylaminoethyl column fractions. An S. derby mutant resistant to lysis by phage OJ367 was isolated. The mutant not only showed decreased receptor activity in vitro when its TCE was tested but had an altered Omp profile. This implied that the 45-kDa Omp is involved as a receptor in coliphage binding; however, this role is affected by the expression of other Omps.
關聯: 38(6), pp.399-408
URI: http://ntour.ntou.edu.tw/handle/987654321/27300
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