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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27299

Title: The Identification of Starch Phosphorylase in the Developing Mungbean (Vigna radiata L.)
Authors: Yuan-Tih Ko;Jin-Yi Chang;Ya-Ting Lee;Yi-Hui Wu
Contributors: NTOU:Department of Food Science
國立臺灣海洋大學:食品科學系
Keywords: Starch phosphorylase;SP;Pho;activity staining;mungbean;Vigna radiata;native-PAGE
Date: 2005
Issue Date: 2011-10-21T02:24:20Z
Publisher: Journal of Agricultural and Food Chemistry
Abstract: Abstract:Starch phosphorylase (SP) in immature mungbean (Vigna radiata L. cv KPS1) seed soluble extract was detected by in situ activity staining and identified by MALDI-TOF mass analysis. After in situ SP assay on native-PAGE, a major starch−enzyme complex was located on the gel zymogram in a dose-dependent manner. This complex depicted two major SP-activity related proteins, 105 kDa and 55 kDa, by SDS−PAGE. The mass and predicted sequence of the tryptic fragments of the isolated 105 kDa protein, analyzed by MALDI-TOF spectroscopy and bioinformatic analysis, confirmed it to be mungbean SP as a result of high similarity to the L-SP of known plant. Polyclonal antibodies raised from the 55 kDa recognized both the 105 kDa and the 55 kDa proteins on the Western blot and neutralized partial SP activity, indicating that the two proteins were immunologically related. The 55 kDa protein possess high similarity to the N-terminal half of the 105 kDa SP was further confirmed. The SP activity and the activity stained protein density in mungbean soluble extract decreased as the seed size increased during early seed growth. These data indicate that mungbean 105 kDa SP and SP activity-related 55 kDa were identified in the developing mungbean.
Relation: 53(14), pp.5708–5715
URI: http://ntour.ntou.edu.tw/handle/987654321/27299
Appears in Collections:[食品科學系] 期刊論文

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