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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27279

Title: Organization and promoter analysis of the zebrafish (Danio rerio) chemokine gene (CXC-64) promoter
Authors: Li-Chen Chen;Jen-Leih Wu;Chyuan-Yuan Shiau;Jyh-Yih Chen
Contributors: NTOU:Department of Food Science
國立臺灣海洋大學:食品科學系
Keywords: Zebrafish;Chemokine;Gene expression;Functional analysis
Date: 2010
Issue Date: 2011-10-21T02:24:13Z
Publisher: Fish Physiology and Biochemistry
Abstract: Abstract:Zebrafish CXC-64, a chemokine representing a superfamily of chemotactic cytokines present in fish, is involved in recruitment, activation, and response to inflammatory stimulation. We cloned and sequenced the genomic DNA of the zebrafish CXC-64 chemokine; it was most similar to CXCL11 from humans and CXCL10 from a catfish. The zebrafish CXC-64 gene is approximately 4.0 kb long and has a four-exon, three-intron structure common to the human CXCL11 gene. However, the promoter region includes a typical TATA box and multi-transcription factor-binding sequences. To understand the roles of lipopolysaccharide (LPS), poly I:poly C, and tumor necrosis factor (TNF)-α in regulating zebrafish CXC-64 expression, serial deletions were made in the promoter region of this clone. Different fragments of the zebrafish CXC-64 5′-flanking region were transfected into RAW264.7 (mouse macrophage; Abelson murine leukemia virus transformed) and zfl (zebrafish liver) cells and then treated with 0, 10, 50, 100, and 200 ng/ml LPS, poly I:poly C, or TNF-α. The results showed that the promoter activity presented dose-dependent effects in LPS-treated RAW264.7 cells, TNF-α-treated RAW264.7 cells, and LPS-treated zfl cells. These results reveal that the zebrafish CXC-64 chemokine gene promoter region can be induced by LPS in both human and fish cell lines, which suggests that it plays an important role in regulating LPS.
Relation: 36(3), pp.511-521
URI: http://ntour.ntou.edu.tw/handle/987654321/27279
Appears in Collections:[食品科學系] 期刊論文

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