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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27267

Title: Rapid PCR-RFLP Method for the Identification of 5 Billfish Species
Authors: Hung-Sheng Hsieh;Tuu-Jyi Chai;Deng-Fwu Hwang
Contributors: NTOU:Department of Food Science
國立臺灣海洋大學:食品科學系
Keywords: PCR-RFLP;billfish;mtDNA;cytochrome b gene;identification
Date: 2005-05-31
Issue Date: 2011-10-21T02:24:10Z
Publisher: Journal of Food Science
Abstract: Abstract:A rapid and reliable polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to identify billfish species Xiphias gladius (swordfish), Makaira nigricans (blue marlin), Makaira indica (black marlin), Istiophorus platypterus (sailfish), and Tetrapturus audax (striped marlin). After DNA extraction and amplifying, the 348-bp PCR products from gene encoding of cytochrome b were subjected to restriction enzyme analysis. No single enzyme tested was able to distinguish the 5 species at the same time, but the combination of results obtained from the digests of Bsa JI and Cac 81 could be used to differentiate the 5 billfish species. This sensitive, rapid, and valid method can be used to detect fraudulent substitutes.
Relation: 70(4), pp,C246-C249
URI: http://ntour.ntou.edu.tw/handle/987654321/27267
Appears in Collections:[食品科學系] 期刊論文

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