English  |  正體中文  |  简体中文  |  Items with full text/Total items : 26988/38789
Visitors : 2325199      Online Users : 53
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27239

Title: IDENTIFICATION OF A STARCH-BRANCHING ENZYME AND COEXISTING STARCH BIOSYNTHETIC ENZYMES FROM PARTIALLY PURIFIED MUNG BEAN (VIGNA RADIATA L.) FRACTIONS
Authors: YUAN-TIH KO;JIN-YI CHANG;CHIEN-CHEN LAI;MAO-RONG CHEN;JIA-WEI CHANG
Contributors: NTOU:Department of Food Science
國立臺灣海洋大學:食品科學系
Date: 2008-02
Issue Date: 2011-10-21T02:24:04Z
Publisher: Journal of Food Biochemistry
Abstract: Abstract:Three starch-branching enzyme (BE) bands (I, II and III) were detected by zymogram in the soluble extract of developing mung bean (Vigna radiata L. cv KPS1), and radioactive method was used to trace BE during purification by liquid chromatography. Protein profiles of bands I and II were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and identified by proteomic analysis with liquid chromatography–mass spectroscopy/mass spectrometry and bioinformatic searching. The 96 kDa BE possessing specific amino acid sequences of the A-type family of BEs was identified from band II. Mung bean 92 kDa sucrose synthase and the previously identified 105 kDa starch phosphorylase (SP) and its 55 kDa fragments were also found in both bands I and II. Western blotting with SP antibodies showed that SP was present in all of the purified BE fractions. In summary, with the aid of proteomic analysis, we have identified a major mung bean BE and coexisting starch biosynthetic enzymes from partially purified fractions.
Relation: 32(1), pp.122–141
URI: http://ntour.ntou.edu.tw/handle/987654321/27239
Appears in Collections:[食品科學系] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML242View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback