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Title: Hepatic Preconditioning Induced Heparin-Binding Epithermal Growth Factor-Like Growth Factor and Amphiregulin in Ischemic Rat Liver
Authors: 張君如
Contributors: NTOU:Department of Food Science
Date: 2010
Issue Date: 2011-10-21T02:24:01Z
Publisher: The 25th Joint Annual Conference of Biomedical Sciences (Taipei, Taiwan. March 27-28, 2010)
Abstract: Abstract:The benefit of ischemic preconditioning (IPC) has been used in hepatic resection and liver transplantation because of increasing the tolerance of hepatic ischemia and decreasing the ischemic-reperfusion injury (IRI). However, the protective mechanisms of IPC are still unclear. The aim of this study was to investigate the role of hepatoprotective proteins in hepatic IRI rat. The hepatic ischemia was induced by using an atraumatic vessel clamp to occlude the left hemi-pedicle of hilum which occluded the portal vein and hepatic artery branches to the median and left hepatic lobes. Then sustained 60 min ischemia was applied for the medial and left lobe. After 60 min ischemia, the experiment was stopped 60 min after reperfusion. The expression levels of hepatoprotective proteins, such as heparin-binding epidermal growth factor-like growth factor (HB-EGF) and amphiregulin (AR), in rat liver were measured by immunohistochemistry. Serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) were examined as markers of hepatocyte injury. The results demonstrated that IRI reduced HB-EGF in rat liver. However, 3 cycles of IPC maintained HB-EGF levels during IPCs and IRI procedures. Besides, 1 and 3 cycles of IPC elevated AR levels in rat liver. Moreover, IRI induced LDH releasing and AST/ALT increasing in rat serum. Nevertheless, the acceleration of these hepatic injury markers was abolished by 1 and 3 cycles of IPC. These findings suggested that IPC may modulate the expression of HB-EGF and AR in liver, and abate the ischemic injury when performing liver resection and liver transplantation.
Relation: Poster no.660 , pp.213
URI: http://ntour.ntou.edu.tw/handle/987654321/27221
Appears in Collections:[食品科學系] 演講及研討會

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