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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27216

Title: Mechanism Studies of Si-Wu-Tang and Ferulic Acid on HER2 Gene Expression of Human Breast Tumor Cells
Authors: Chun-Ju Chang
Contributors: NTOU:Department of Food Science
Keywords: Si-Wu-Tang;ferulic acid;breast tumor;HER2;estrogen receptor;signal transduction
Date: 2006
Issue Date: 2011-10-21T02:24:00Z
Publisher: (Doctoral Dissertation)
Abstract: Abstract:The consumption of over-the-counter (OTC) natural products in perimenopausal women and patients with breast diseases remains a challenging problem. Previous studies have shown that the proliferation of breast cancer cells could be enhanced by Angelica sinensis Radix, ginsenoside Rg1, and red clover extracts. Therefore, the safety of OTC natural products required caution. Si-Wu-Tang (SWT) consists of Angelica sinensis Radix, Rehmanniae preparata Radix, Ligusticum chuanxiong Hortorum, and Paeonia lactiflora Pallas. It is a well-known Chinese formula for the treatment of gynecologic diseases, but the efficacy and mechanisms of SWT on breast disease have never been studied. The aims of this study were to investigate the growth-stimulation effect of SWT and its constituents on MCF-7 breast cancer cells and the SWT-modulated cell signaling as well as the HER2 gene expression. Using primary fibroadenoma culture and breast cancer cell lines, MCF-7 (estrogen receptor-positive; ER+, HER2-low), BT-474 (ER+, HER2-high), MDA-MB-231 (ER-, HER2-low) and SK-BR-3 (ER-, HER2-high), as in vitro models, the mitogenic effects of SWT, its constituents, and its active compound, ferulic acid (FA), were assessed by trypan blue dye exclusion assay and DNA flow cytometry. Besides, SWT-modulated cell signaling and HER2 gene expression were analysed by Western blot and real-time RT-PCR. In addition, by oophorectomized athymic BALB/c nude mice as in vivo model, MCF-7 cells were implanted in subcutaneous sites of mice. The effects of Si-Wu-Tang on tumor growth and uterus weight were assessed after 21 days of treatment. The results showed that SWT enhanced cell proliferation on primary fibroadenoma culture and breast cancer cells in a dose-dependent manner. Water extract of Angelica sinensis and ethanol extract of Ligusticum chuanxiong played key roles on SWT-induced proliferative effect on MCF-7 cells. Meanwhile, HER2 downstream signaling molecules, AKT and ERK1/2, were involved in SWT-enhance
Relation: pp.1-140
URI: http://ntour.ntou.edu.tw/handle/987654321/27216
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