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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27155

Title: Effect of Mitochondrial Stress on Nuclear Gene Expression
Authors: Chang CJ;Wang CH;Hung WY;Yin PH;Wei YH;Chi CW;Lee HC
Contributors: NTOU:Department of Food Science
Date: 2007
Issue Date: 2011-10-21T02:23:46Z
Publisher: The 4th Conference of Asian Society of Mitochondrial Research and Medicine (Seoul, Korea. Feb. 2-3, 2007)
Abstract: Abstract:Mitochondrial genome instability and reduced copy number of mitochondrial DNA (mtDNA) were commonly found in human cancers. We proposed that somatic alterations in mtDNA could promote cancer progression through changes in nuclear gene expression. The aim of this study is to investigate the effect of mitochondrial dysfunction on nuclear DNA-encoded gene expression in cancer cells. We treated human cancer cells, such as hepatoma HA22T/VGH and HepG2 cells, gastric cancer SCM1 cells, as well as prostate cancer LNCaP cells, with Ethidium Bromide for 5-7 days to partially deplete the copy number of mtDNA (mitochondrial stress). By using human whole genome cDNA microarrays, Affymetrix U133 plus 2 and Applied Biosystems AB1700, the gene expression profiles changed by the mitochondrial stress were observed in these cells. We found 471, 193, 155 and 192 genes were changed more then three-fold in HA22T, HepG2, SCM1 and LNCaP cells, respectively. Meanwhile, about one-third of these genes were novel gene; several genes were simultaneously up- or down-regulated by the mitochondrial stress in four cell lines. It’s noteworthy that NADH dehydrogenase 5 and 6, involved in oxidative phosphorylation, were dramatically down-regulated in this kind of mitochondrial stress. Moreover, we were using online tools, NetAffx and PANTHER, to refer Gene Ontology, KEGG and GenMAPPs databases to reveal the pathway candidates affected by the mitochondrial stress. As shown in NetAffx and PANTHER, the mitochondrial stress may affected biological processes, including mitochondrial electron transport, neurotransmitter transport, apoptosis, cell cycle, GTPase mediated signal transduction, amino acid biosynthesis, protein ubiquitination, lipid metabolism and regulation of transcription from RNA polymerase II promoter, etc.. In addition, cellular signaling pathway like oxidative stress response, angiogenesis, Wnt signaling, p53 pathway, and inflammation mediated by chemokine and cytokine signaling pathway
Relation: Poster no.8, pp.114
URI: http://ntour.ntou.edu.tw/handle/987654321/27155
Appears in Collections:[食品科學系] 演講及研討會

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