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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27146

Title: Application of PCR-RFLP analysis on species identification of canned tuna
Authors: Wen-Feng Lin;Deng-Fwu Hwang
Contributors: NTOU:Department of Food Science
Keywords: PCR-RFLP;Canned tuna;Species identification;Cytochrome b gene;Magnetic beads extraction
Date: 2007-09
Issue Date: 2011-10-21T02:23:44Z
Publisher: Food Control
Abstract: Abstract:The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was developed to identify the species of Thunnus thynnus, T. alalunga, T. obesus, T. albacares, Euthynnus pelamis (Katsuwonus pelamis), E. affinis, Auxis thazard and Sarda orientalis in products of canned tuna. To increase the efficiency and sensitivity of DNA extraction, a recent developed method of binding magnetic beads was applied in this study. Two sets of primer were designed to amplify 126 bp and 146 bp of partial mitochondrial cytochrome b gene, and five restriction enzymes including Bsp1286I, HincII, RsaI, ScaI and MboII were determined to analyze the short length fragments. The developed PCR-RFLP method was applied to authenticate species of 18 commercial canned tuna successfully, and it really provided a useful and academic technique to identify eight species in canned tuna.
Relation: 18(9), pp.1050-1057
URI: http://ntour.ntou.edu.tw/handle/987654321/27146
Appears in Collections:[食品科學系] 期刊論文

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