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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27134

Title: A PCR-ELISA Method for Direct Detection of the Oyster Pathogen Haplosporidium nelsoni
Authors: Yuan-Tih Ko;Marion Man-Ying Chan;Susan E. Ford;Dunne Fong
Contributors: NTOU:Department of Food Science
Date: 1999-03
Issue Date: 2011-10-21T02:23:41Z
Publisher: Journal of Marine Biotechnology
Abstract: Abstract:A rapid method, utilizing both polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), was developed for detection of oyster MSX disease. The technique included using Haplosporidium nelsoni pathogen-specific PCR primers (based on ribosomal RNA genes), a Chelex resin (for rapid DNA extraction from oyster mantle tissues), and cloned H. nelsoni rRNA plasmid DNA (for use as a capture probe). Digoxigenin was incorporated into the pathogen-specific PCR products, which were captured by the coated probe in a fast hybridization reaction and then detected by ELISA. The sensitivity of PCR amplification on cloned plasmid DNA was 10 fg for detection by stained agarose gel, and increased to 0.01 fg for ELISA. Positive signals were observed in infected oysters using the PCR-ELISA technique. This method may be applicable to early detection of infection.
Relation: 1(2), pp.147-154
URI: http://ntour.ntou.edu.tw/handle/987654321/27134
Appears in Collections:[食品科學系] 期刊論文

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