National Taiwan Ocean University Institutional Repository:Item 987654321/27133
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题名: Morphology, Associated Protein Analysis, and Identification of 58-kDa Starch Synthase in Mungbean (Vigna radiata L. cv. KPS1) Starch Granule Preparations
作者: Yuan-Tih Ko;Yu-Ling Dong;Ying -Fang Hsieh;Ja-Chi Kuo
贡献者: NTOU:Department of Food Science
國立臺灣海洋大學:食品科學系
关键词: Vigna radiata;starch granule;granule morphology;granule-associated protein;granule bound starch synthase
日期: 2009
上传时间: 2011-10-21T02:23:41Z
出版者: J Agric Food Chem
摘要: Abstract:Raw starch granules of mature mungbean (Vigna radiata L. cv. KPS1) seeds were prepared by two methods into crude and cesium chloride (CsCl)-washed forms. The purity, shape, size distribution, and associated protein profiles were examined. The appearance of raw starch granules showed a bimodal type distribution in which average granules had typical ovoid shapes, whereas the small ones were spherical. Abnormal granule surface with distinct tumor-like or dented hole features were also observed in raw starch granules. CsCl-washed granules had a smooth surface compared to that of the crude form. The granule size distribution ranged from 6−35 μm; most 15−25 μm (53%), followed by 25−35 μm (26%). Small granules (<15 μm) amounted to 18%, and granules >35 μm consisted of 3%. The two forms were further refined by trichloroacetic (TCA) treatment to reveal surface proteins on the crude granules or tightly bound proteins on CsCl-washed granules. In the washed-refined granules, only a few integral proteins were retained. The major 58-kDa protein was identified to be granule-bound starch synthase I by sequence homology with that in cowpea (Vigna unguiculata) and maize (Zea mays) using MALDI-TOF mass and Mascot search.
關聯: 57(10), pp.4426-4432
URI: http://ntour.ntou.edu.tw/handle/987654321/27133
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