English  |  正體中文  |  简体中文  |  Items with full text/Total items : 27308/39152
Visitors : 2447319      Online Users : 45
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27133

Title: Morphology, Associated Protein Analysis, and Identification of 58-kDa Starch Synthase in Mungbean (Vigna radiata L. cv. KPS1) Starch Granule Preparations
Authors: Yuan-Tih Ko;Yu-Ling Dong;Ying -Fang Hsieh;Ja-Chi Kuo
Contributors: NTOU:Department of Food Science
國立臺灣海洋大學:食品科學系
Keywords: Vigna radiata;starch granule;granule morphology;granule-associated protein;granule bound starch synthase
Date: 2009
Issue Date: 2011-10-21T02:23:41Z
Publisher: J Agric Food Chem
Abstract: Abstract:Raw starch granules of mature mungbean (Vigna radiata L. cv. KPS1) seeds were prepared by two methods into crude and cesium chloride (CsCl)-washed forms. The purity, shape, size distribution, and associated protein profiles were examined. The appearance of raw starch granules showed a bimodal type distribution in which average granules had typical ovoid shapes, whereas the small ones were spherical. Abnormal granule surface with distinct tumor-like or dented hole features were also observed in raw starch granules. CsCl-washed granules had a smooth surface compared to that of the crude form. The granule size distribution ranged from 6−35 μm; most 15−25 μm (53%), followed by 25−35 μm (26%). Small granules (<15 μm) amounted to 18%, and granules >35 μm consisted of 3%. The two forms were further refined by trichloroacetic (TCA) treatment to reveal surface proteins on the crude granules or tightly bound proteins on CsCl-washed granules. In the washed-refined granules, only a few integral proteins were retained. The major 58-kDa protein was identified to be granule-bound starch synthase I by sequence homology with that in cowpea (Vigna unguiculata) and maize (Zea mays) using MALDI-TOF mass and Mascot search.
Relation: 57(10), pp.4426-4432
URI: http://ntour.ntou.edu.tw/handle/987654321/27133
Appears in Collections:[食品科學系] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML237View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback