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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27123

Title: Detection of Proteins Related to Starch Synthase Activity in the Developing Mungbean (Vigna radiata L.)
Authors: Yuan-Tih Ko;Chun-Hsu Pan;Ya-Ting Lee;Jin-Yi Chang
Contributors: NTOU:Department of Food Science
Keywords: Starch synthase;activity staining;SS;isoelectric focusing;mungbean;Vigna radiata;native-PAGE
Date: 2005
Issue Date: 2011-10-21T02:23:38Z
Publisher: Journal of Agricultural and Food Chemistry
Abstract: Abstract:Proteins associated with starch synthase (SS) activities were identified in immature mungbeans (Vigna radiata L. cv KPS1). Seed soluble extract was separated by native-PAGE and subjected to in situ activity staining. The gel zymogram located starch−enzyme complex bands. The soluble extract was also partitioned by preparative-IEF and screened for SS activity using radioactive assay. IEF fractions eluted within pH 4−6 revealed enriched SS activity of 145-fold. Parallel comparison of the protein profiles among the activity stained enzyme complex and the active isoelectric focused fractions on SDS−PAGE depicted three SS-activity-related proteins with molecular size of 32, 53, and 85 kDa. The 85 kDa protein, however, was identified to be methionine synthase by MALDI-TOF analysis and should be a protein physically associated with the active SS. Polyclonal antibodies raised from eluted native enzyme complex neutralized up to 90% activity and antigenically recognize the other 53 and 32 kDa proteins on Western blot. Antibodies raised from the two individual denatured proteins were able to neutralize SS activities near 60% separately, indicating that the 53 kDa and 32 proteins associated with SS activity are potentially involved in starch biosynthesis during mungbean seed development.
Relation: 53(12), pp.4805–4812
URI: http://ntour.ntou.edu.tw/handle/987654321/27123
Appears in Collections:[食品科學系] 期刊論文

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