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题名: Lemon ascorbate peroxidase: cDNA cloning and biochemical characterization
作者: Ya-Han Dai;Chich-Yu Huang;Lisa Wen;Dey-Chyi Sheu;Chi-Tsai Lin
贡献者: NTOU:Institute of Bioscience and Biotechnology
國立臺灣海洋大學:生物科技研究所
关键词: Ascorbate peroxidase;Citrus limon;Saccharomy cescerevisiae;Three-dimension structural model
日期: 2012-01-01
上传时间: 2011-10-21T02:22:45Z
出版者: Botanical Studies
摘要: Abstract:Ascorbate peroxidase (Apx) plays important roles both as a reductant and as a H2O2 scavenger via ascorbate (AsA). In this paper, we discuss how a ClApx cDNA (1,068 bp, GQ465430) encoding a putative Apx was cloned from lemon (Citrus limon). The deduced amino acid sequence is similar to the Apxes from other plant species. A 3-D structural model of ClApx was constructed based on the crystal structure of Pisum sativum Apx (PDB code 1APX). To characterize the ClApx protein, the coding region was subcloned into an expression vector pYEX-S1 and transformed into Saccharomyces cerevisiae. The recombinant His6-tagged ClApx was overexpressed and purified by Ni(superscript 2+) -nitrilotriacetic acid Sepharose. The purified enzyme showed two prominent bands on 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The Michaelis constant (K(subscript M)) values of the recombinant enzyme for AsA and H2O2 were 0.40 and 0.11 mm, respectively. The enzyme was active from pH range 6 to 8. The thermal inactivation of the enzyme showed a half-life of 6.5 min at 45°C, and its inactivation rate constant K(subscript i) was 1.1 × 10(superscript -1) min(superscript -1). The enzyme retained 35% activity after chymotrypsin digestion at pH 8 and 37°C for 40 min.
關聯: 53(1), pp.11-19
URI: http://ntour.ntou.edu.tw/handle/987654321/27109
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