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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27074

Title: Noninvasive harmonics optical microscopy for long-term observation of embryonic nervous system development in vivo
Authors: Szu-Yu Chen;Cho-Shuen Hsieh;Shi-Wei Chu;Cheng-Yung Lin;Ching-Yi Ko;Yi-Chung Chen;Huai-Jen Tsai;Chin-Hwa Hu;Chi-Kuang Sun
Contributors: NTOU:Institute of Bioscience and Biotechnology
國立臺灣海洋大學:生物科技研究所
Keywords: 3RD-HARMONIC GENERATION MICROSCOPY;ENDOGENOUS STRUCTURAL PROTEINS;2ND-HARMONIC GENERATION;FLUORESCENCE MICROSCOPY;MULTIPHOTON MICROSCOPY;COHERENCE TOMOGRAPHY;BRAIN-TISSUE;ZEBRAFISH;DYNAMICS;BIOLOG
Date: 2006-10-30
Issue Date: 2011-10-21T02:22:32Z
Publisher: Journal of Biomedical Optics
Abstract: Abstract:Nervous system development is a complicated dynamic process, and many mechanisms remain unknown. By utilizing endogenous second-harmonic-generation as the contrast of polarized nerve fibers and third-harmonic-generation (THG) to reveal morphological changes, we have successfully observed the vertebrate embryonic nervous development from the very beginning based on a 1230-nm light source. The dynamic development of the nerve system within a live zebrafish embryo can be recorded continuously more than 20hr without fluorescence markers. Since the THG process is not limited by the time of gene expression and differentiation as fluorescence-based techniques are, the observable stages can be advanced to the very beginning of the development process. The complete three-dimensional brain development from a neural plate to a neural tube can be uncovered with a submicron lateral resolution. We have, for the first time, also reported the generation of SHG from myelinated nerve fibers and the outer segment of the photoreceptors with a stacked membrane structure. Our study clearly indicates the fact that higher-harmonics-based optical microscopy has the strong potential to long-term in vivo study of the nervous system, including genetic disorders of the nervous system, axon pathfinding, neural regeneration, neural repair, and neural stem cell development.
Relation: 11(5)/054022
URI: http://ntour.ntou.edu.tw/handle/987654321/27074
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

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