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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27067

Title: Monoclonal anti-double-stranded DNA antibodies cross-react with phosphoglycerate kinase 1 and inhibit the expression and production of IL-2 in activated Jurkat T cell line
Authors: Hui-Ying Luan;Shye-Jye Tang;Winnie Yang;Chang-Youh Tsai;Guang-Huan Sun;Kuang-Hui Sun
Contributors: NTOU:Institute of Bioscience and Biotechnology
Keywords: Anti-double strand DNA antibodies;IL-2;Phosphoglycerate kinase 1;SLE
Date: 2006-09
Issue Date: 2011-10-21T02:22:30Z
Publisher: Clinical Immunology
Abstract: Abstract:Anti-double strand DNA antibodies (anti-dsDNA) involve in lupus nephritis. However, their role in tissue damage mechanism remains unclear. In this study, a 45-kDa cognate antigen of anti-dsDNA monoclonal antibodies 9D7 was identified by two-dimensional gel electrophoresis and determined to be human phosphoglycerate kinase 1 (PGK-1) by MALDI-TOF analysis. The binding of 9D7 to PGK-1 was not affected by DNase I but was inhibited by thymus dsDNA. Human SLE sera with high anti-dsDNA titers had a high affinity with PGK. In activated Jurkat T cells, 9D7 decreased the PGK-1 mRNA production and IL-2 promoter activity. Reduction in IL-2 gene expression and protein production were observed in the 9D7-treated cells. Because PGK-1 deficiency may cause mental tardy and hemolytic anemia, interaction between anti-dsDNA and PGK-1 may be important in lupus pathogenesis. Moreover, reduction in IL-2 production by anti-dsDNA suggests their role in increasing infection rate and decreasing proper generation of activation-induced cell death.
Relation: 120(3), pp.326–334
URI: http://ntour.ntou.edu.tw/handle/987654321/27067
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

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