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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27065

Title: Monoclonal ribosomal P autoantibody inhibits the expression and release of IL-12, TNF-α and iNOS in activated RAW macrophage cell line
Authors: Kuang-Hui Sun;Shye-Jye Tang;Chau-Yaun Chen;Tai-Ping Lee;Chi-Kuang Feng;Chia-Li Yu;Guang-Huan Sun
Contributors: NTOU:Institute of Bioscience and Biotechnology
Keywords: Anti-P autoantibody;Interleukin-12;Macrophage;Systemic lupus erythematosus;Tumor necrosis factor α;Interleukin-10
Date: 2005-03
Issue Date: 2011-10-21T02:22:30Z
Publisher: Journal of Autoimmunity
Abstract: Abstract:Monoclonal ribosomal P protein antibody (anti-P mAb) may bind to the cell surface, penetrate into cells, and induce apoptosis of Jurkat T cells. Recently, modulation of cytokines has been considered to be important in the pathogenesis of systemic lupus erythematous (SLE). In this study, effects of anti-P mAbs (9B6) on gene expression of cytokines, apoptosis, and reactive oxygen species in murine macrophage RAW 264.7 were analyzed by RT–PCR and ELISA and those on IL-12 promoter activity was determined in an IL-12p40 promoter-reporter gene transfected cell line RAW (IL-12p40-SEAP). After treating LPS-activated RAW 264.7 with 9B6 for 6 or 24 h, the levels of mRNA and protein expression of IL-12, TNF-α, and iNOS were significantly inhibited by 25%, 16%, and 13%, respectively. The IL-12 promoter activity of RAW (IL-12p40-SEAP) was also inhibited by 13–22%. However, inhibitory effects were not observed in cells pre-treated with IgG1 for 1 h. The productions of IL-10 in LPS-activated RAW 264.7 and human macrophages were potentiated by 9B6 up to 65% and 51%, respectively. Since anti-P Abs inhibit productions of IL-12 and TNF-α and enhance IL-10 production in macrophages, these autoantibodies may augment Th2 responses and amplify lupus manifestations by causing immunological polarity and lymphocyte dysfunction.
Relation: 24(2), pp.135–143
URI: http://ntour.ntou.edu.tw/handle/987654321/27065
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

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