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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27063

Title: ENCAPSULATION AND GROWTH CHARACTERISTICS OF THREE DIFFERENT CELLS IN ALGINATE GEL BEADS CONTAINING RECONSTITUTED COLLAGEN FIBERS
Authors: SHIAO-WEN TSAI;FU-YIN HSU;YNG JIIN WANG
Contributors: NTOU:Institute of Bioscience and Biotechnology
國立臺灣海洋大學:生物科技研究所
Keywords: collagen
Date: 2006-04-25
Issue Date: 2011-10-21T02:22:29Z
Publisher: Biomedical Engineering: Applications, Basis and Communications
Abstract: Abstract:Alginate gel beads containing reconstituted collagen fibers were fabricated and used to culture three different cells - GH3 pituitary tumor cells, L929 fibroblasts and rat calvaria osteoblasts. These cells when entrapped within the collagen-containing alginate behaved differently from those grown in the alginate matrix. Of the three cells tested, the existence of collagen fibers is most beneficial for the viability and proliferation of GH3 cells which can grow either as suspended or attached to the matrix. For the case of osteoblasts derived from the primary culture of rat calvaria, most of the cells entrapped in alginate lost their viability after seeding. The collagen fibers in the alginate gel beads nevertheless decelerated the apotosis process. Furthermore, the entrapped osteoblasts were capable of migrating out of the collagen-containing alginate matrix when the alginate gel beads were in contact with the culture dish. The mineralization of matrix, a key phenotypic expression of osteoblatic cells, was shown by von-Kosa staining on the culturing dish after 14 days cultivation of the cells which crawled out of the gel beads. The collagen-containing alginate beads appear to be more versatile than the conventional alginate gel beads for biomedical applications.
Relation: 18(2), pp.62-66
URI: http://ntour.ntou.edu.tw/handle/987654321/27063
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

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