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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27061

Title: Iridovirus Bcl-2 protein inhibits apoptosis in the early stage of viral infection
Authors: Pei-Wen Lin;Yi-Jen Huang;Joseph Abraham Christopher John;Ya-Nan Chang;Chung-Hsiang Yuan;Wen-Ya Chen;Chiao-Hwa Yeh;San-Tai Shen;Fu-Pang Lin;Wen-Huei Tsui
Contributors: NTOU:Institute of Bioscience and Biotechnology
Keywords: Apoptosis;Bcl-2;Grouper;Iridovirus;Mitochondria
Date: 2008-01
Issue Date: 2011-10-21T02:22:29Z
Publisher: Apoptosis
Abstract: Abstract:The grouper iridovirus (GIV) belongs to the family Iridoviridae, whose genome contains an antiapoptotic B-cell lymphoma (Bcl)-2-like gene. This study was carried-out to understand whether GIV blocks apoptosis in its host. UV-irradiated grouper kidney (GK) cells underwent apoptosis. However, a DNA fragmentation assay of UV-exposed GK cells after GIV infection revealed an inhibition of apoptosis. The UV- or heat-inactivated GIV failed to inhibit apoptosis, implying that a gene or protein of the viral particle might contribute to an apoptosis inhibitory function. The DNA ladder assay for GIV-infected GK cells after UV irradiation confirmed that apoptosis inhibition was an early process which occurred as early as 5 min post-infection. A GIV-Bcl sequence comparison showed distant sequence similarities to that of human and four viruses; however, all possessed the putative Bcl-2 homology (BH) domains of BH1, BH2, BH3, and BH4, as well as a transmembrane domain. Northern blot hybridization showed that GIV-Bcl transcription began at 2 h post-infection, and the mRNA level significantly increased in the presence of cycloheximide or aphidicolin, indicating that this GIV-Bcl is an immediate-early gene. This was consistent with the Western blot results, which also revealed that the virion carries the Bcl protein. We observed the localization of GIV-Bcl on the mitochondrial membrane and other defined intracellular areas. By immunostaining, it was proven that GIV-Bcl-expressing cells effectively inhibited apoptosis. Taken together, these results demonstrate that GIV inhibits the promotion of apoptosis by GK cells, which is mediated by the immediate early expressed viral Bcl gene.
Relation: 13(1), pp.165-176
URI: http://ntour.ntou.edu.tw/handle/987654321/27061
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

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