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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27052

Title: Highly Selective DNA-Based Sensor for Lead(II) and Mercury(II) Ions
Authors: Chi-Wei Liu;Chih-Ching Huang;Huan-Tsung Chang
Contributors: NTOU:Institute of Bioscience and Biotechnology
國立臺灣海洋大學:生物科技研究所
Date: 2009
Issue Date: 2011-10-21T02:22:27Z
Publisher: Analtical Chemistry
Abstract: Abstract:We have developed a technique for the highly selective and sensitive detection of Pb2+ and Hg2+ using a thrombin-binding aptamer (TBA) probe labeled with the donor carboxyfluorescein (FAM) and the quencher 4-([4-(dimethylamino)phenyl]azo)benzoic acid (DABCYL) at its 5′ and 3′ termini, respectively. The TBA has a random coil structure that changes into a G-quartet structure and a hairpin-like structure upon binding Pb2+ and Hg2+ ions, respectively. As a result, the fluorescence decreases through fluorescence resonance energy transfer (FRET) between the fluorophore and quencher. These changes in fluorescence intensity allow the selective detection of Pb2+ and Hg2+ ions at concentrations as low as 300 pM and 5.0 nM using this TBA probe in the presence of phytic acid and a random DNA/NaCN mixture, respectively. The linear correlation existed between the fluorescence intensity and the concentration of Pb2+ and Hg2+ over the range of 0.5−30 nM (R2 = 0.98) and 10−200 nM (R2 = 0.98), respectively. To the best of our knowledge, this is the first example of a single DNA-based sensor that allows the detection of both Hg2+ and Pb2+ ions. This simple and cost-effective probe was also applied to separately determine Pb2+ in soil samples and spiked Hg2+ in pond samples.
Relation: 81(6), pp.2383–2387
URI: http://ntour.ntou.edu.tw/handle/987654321/27052
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

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