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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27046

Title: In vivo long-term continuous observation of gene expression in zebrafish embryo nerve systems by using harmonic generation microscopy and morphant technology
Authors: Cho-Shuen Hsieh;Ching-Yi Ko;Szu-Yu Chen;Tzu-Ming Liu;Jian-Shiung Wu;Chin-Hwa Hu;Chi-Kuang Sun
Contributors: NTOU:Institute of Bioscience and Biotechnology
國立臺灣海洋大學:生物科技研究所
Keywords: in vivo imaging;harmonic generation microscopy;third harmonic generation;second harmonic generation;three-photon excited fluorescence;embryology;gene expression;morphant technolog
Date: 2008-12-30
Issue Date: 2011-10-21T02:22:26Z
Publisher: Journal of Biomedical Optics
Abstract: Abstract:Gene expression plays an important role in embryo development and organ function. Previous studies have shown that harmonic generation microscopy (HGM) can be used as a fluorescence signal-independent, minimally invasive method with a subcellular 3-D resolution and a penetration depth in the order of millimeters for long-term continuous imaging of vertebrate embryos. We show that it is ideal to combine in vivo HGM with the morphant technology for minimally invasive, long-term continuous observation of gene expression in the nervous system of vertebrate embryos. Since second- and third-harmonic generations (SHG, THG) are virtual-state-transition-based systems that depend only on the structure of the organisms, they are not temporally limited by the expression of the fluorescence proteins. We successfully identified the expression of the zarnt2a and the hif-1α, 2α, and 3α genes in the nervous system of zebrafish embryos with specific knockdown genes by microscopically observing the embryos from the early stages of embryogenesis. The results from a combination of the two different modalities, i.e., SHG microscopy and THG microscopy, successfully revealed the weak cell adhesion, cell apoptosis, nerve formation reduction, and neural tube distortion in the morphant zebrafish embryos.
Relation: 13(6), pp.064041
URI: http://ntour.ntou.edu.tw/handle/987654321/27046
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

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