National Taiwan Ocean University Institutional Repository:Item 987654321/27039
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題名: Biochemical characterization of a novel 2-Cys peroxiredoxin from Antrodia camphorata
作者: Jenq-Kuen Huang;Chuian-Fu Ken;Hui-Ming Huang;Chi-Tsai Lin
貢獻者: NTOU:Institute of Bioscience and Biotechnology
國立臺灣海洋大學:生物科技研究所
關鍵詞: Mushroom;Antrodia camphorata;Expression;2-Cys Peroxiredoxin (2-Cys Prx)
日期: 2007-02
上傳時間: 2011-10-21T02:22:24Z
出版者: Applied Microbiology and Biotechnology
摘要: Abstract:Peroxiredoxins (Prxs) play important roles in antioxidation and cell signaling. A gene encoding a novel 2-Cys Prx was identified based on sequence homology in an expressed sequence tag database of the Antrodia camphorata, a medicinal mushroom found only in Taiwan. The 2-Cys Prx cDNA (940 bp) encodes a protein of 188 amino acid residues with calculated molecular mass of 20,965 Da and a pI of 5.89. The coding region was subcloned into pAVD10, transformed into Escherichia coli, and expressed as a His-tagged fusion protein. The purified enzyme was characterized under various conditions. The Prx retained 68% activity after being heated at 60°C for 2 min. It was stable under a broad pH range from 5 to 11. The enzyme activity was slightly decreased in the presence of 1% sodium dodecyl sulfate. The enzyme was somewhat susceptible to chymotrypsin treatment but resistant to digestion by trypsin.
關聯: 74(1), pp.84-92
URI: http://ntour.ntou.edu.tw/handle/987654321/27039
顯示於類別:[生命科學暨生物科技學系] 期刊論文

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