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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27038

Title: Biochemical Characterization of a Catalase from Antrodia Camphorata: Expression in Escherichia coli and Enzyme Properties
Authors: Chuian-Fu Ken;Hsueh-Tai Chen;Reny-Chang Chang;Chi-Tsai Lin
Contributors: NTOU:Institute of Bioscience and Biotechnology
國立臺灣海洋大學:生物科技研究所
Keywords: Antrodia camphorata;Catalase;Expression
Date: 2008-04-01
Issue Date: 2011-10-21T02:22:24Z
Publisher: Botanical Studies
Abstract: abstract:Catalase plays important roles in antioxidation and cell signaling. One cDNA (1794bp, DQ021914) encoding the putative catalase was cloned from Antrodia camphorata. The deduced amino acid sequence is conserved among the reported catalases. To characterize the A. camphorata catalase, the coding region was subcloned into a vector pET-20b(+) and transformed into E. coli. The recombinant 6His-tagged catalase was expressed and purified by Ni(superscript 2+)-nitrilotriacetic acid sepharose. The purified enzyme showed one band by 10% SDS-PAGE. The enzyme retained 50% activity at 60℃ for 14 min. The enzyme was active under a broad pH range from 7.8 to 11.2. The enzyme showed 67% activity after 4h of incubation at 37℃ with trypsin. It was also proven able to protect intact supercoiled plasmid DNA from •OH-induced nicking. Study of the enzyme's properties may prove beneficial for future applications in medicine or health food.
Relation: 49(2), pp.119-125
URI: http://ntour.ntou.edu.tw/handle/987654321/27038
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

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