English  |  正體中文  |  简体中文  |  Items with full text/Total items : 26988/38789
Visitors : 2349623      Online Users : 32
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27037

Title: Biochemical Characterization of a Cambialistic Superoxide Dismutase Isozyme from Diatom Thallassiosira weissflogii: Cloning, Expression, and Enzyme Stability
Authors: Jenq-Kuen Huang;Lisa Wen;Hsu Ma;Zong-Xian Huang;Chi-Tsai Lin
Contributors: NTOU:Institute of Bioscience and Biotechnology
國立臺灣海洋大學:生物科技研究所
Keywords: Thallassiosira weissflogii;expression;cambialistic superoxide dismutase (Fe/Mn−SOD)
Date: 2005
Issue Date: 2011-10-21T02:22:24Z
Publisher: Journal of Agricultural and Food Chemistry
Abstract: Abstract:A cDNA clone of 1081 bp encoding a second putative superoxide dismutase (SOD) from diatom Thallassiosira weissflogii was cloned by the polymerase chain reaction technique. The cDNA encodes a protein of 286 amino acid residues. Alignment of the truncated SOD sequence containing 217 amino acid residues with Mn−SODs from Vibrio mimicus and Escherichia coli, as well as two Fe−SODs from E. coli and Photobacterium leiognathi, this SOD showed greater homology to Mn−SOD. The residues required to coordinate the manganese ion were conserved in all reported Mn−SOD. The recombinant SOD has a half life of deactivation of 14.7 min at 65 °C. Its thermal inactivation rate constant Kd was 3.21 × 10-2 min-1. The enzyme was stable in a broad pH range from 4 to 12. The presence of imidazole (up to 0.8 M) and sodium dodecylsulfate (up to 4%) had little effect on the enzyme's activity. The atomic absorption spectrometric assay showed the presence of 0.3 atom of iron/manganese (2:1) in each SOD subunit. Reconstituted activity suggested that diatom SOD was cambialistic Fe/Mn−SOD.
Relation: 53(16), pp.6319–6325
URI: http://ntour.ntou.edu.tw/handle/987654321/27037
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML340View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback