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http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27035
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| Title: | Comparative Study on Antibacterial Effect of Pediocin ACCEL and Nisin Against Fluorescencestained Listeria monocytogenes BCRC 14845 |
| Authors: | Chien-Wei Wu;Li-Jung Yin;Todd Hsu;Shann-Tzong Jiang |
| Contributors: | NTOU:Institute of Bioscience and Biotechnology
國立臺灣海洋大學:生物科技研究所 |
| Keywords: | bacteriocin;nisin;pediocin;Listeria monocytogenes |
| Date: | 2005-01
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| Issue Date: | 2011-10-21T02:22:23Z
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| Publisher: | Journal of Food Science |
| Abstract: | Abstract:Effects of pediocin ACCEL (Class IIa, pediocin-like bacteriocins) and nisin on carboxyfluorescein diacetate (cFDA) -stained Listeria monocytogenes BCRC 14845 was carried out. Decrease of fluorescence intensity in stained cells and increase in efflux were observed with the increase in bacteriocin concentrations up to 12.5 μg/mL. No further decreases in fluorescence intensity and cell numbers were obtained when the concentration of bacteriocins was over 12.5μg/mL. The leakage of efflux was further confirmed by the release of intracel-lular ultraviolet-absorbing substances. Comparing the leaking time of fluorescence, the intensity of cFDA in nisin-treated cells within 1 min incubation was almost comparable to that in pediocin-treated samples after 40 min incubation. Considering the leakage of cFDA, UV-absorbing substances, changes in cell numbers during incubation of cFDA-stained cells, and restoration ability, nisin seemed to reveal mainly bacteriostatic effect and partially bactericidal effect on L. monocytogenes and to cause easier-to-repair membrane damage. However, pediocin ACCEL appeared to have both bacteriostatic and bactericidal effects on L. monocytogenes and to cause more difficult-to-repair membrane damage. |
| Relation: | 70(1), pp.M75–M80 |
| URI: | http://ntour.ntou.edu.tw/handle/987654321/27035 |
| Appears in Collections: | [生命科學暨生物科技學系] 期刊論文
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