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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27031

Title: Colorimetric determination of urinary adenosine using aptamer-modified gold nanoparticles
Authors: Shih-Ju Chen;Yu-Fen Huang;Chih-Ching Huang;Kun-Hong Lee;Zong-Hong Lin;Huan-Tsung Chang
Contributors: NTOU:Institute of Bioscience and Biotechnology
國立臺灣海洋大學:生物科技研究所
Keywords: G-quartet;Aptamer;Gold nanoparticle;Sensor;Adenosine triphosphate (ATP);Urine sample
Date: 2008-06-15
Issue Date: 2011-10-21T02:22:22Z
Publisher: Biosensors and Bioelectronics
Abstract: Abstract:This paper describes a colorimetric sensing approach for the determination of adenosine triphosphate (ATP) using aptamer-modified gold nanoparticles (Apt-Au NPs). In the absence of the analytes, the color of the Apt-Au NPs solution changed from wine-red to purple as a result of salt-induced aggregation. Binding of the analytes to the Apt-Au NPs induced folding of the aptamers on the Au NP surfaces into four-stranded tetraplex structures (G-quartet) and/or an increase in charge density. As a result, the Apt-Au NPs solution was wine-red in color in the presence of the analytes under high salt conditions. For mixtures of ATP (20.0–100.0 nM), Apt-Au NPs (3.0 nM), 10.0% poly(ethylene glycol), 0.2 μM TOTO-3, 150.0 mM NaCl, 15.0 mM KCl, and 16.0 mM Tris–HCl (pH 7.4), a linear correlation (R2 = 0.99) existed between the ratio of the extinctions of the Apt-Au NPs at 650 and 520 nm (Ex650/520) and the concentration of ATP. The limit of detection for ATP was 10.0 nM. The practicality of this simple, sensitive, specific, and cost-effective approach was demonstrated through the determination of the concentration of adenosine in urine samples.
Relation: 23(11), pp.1749–1753
URI: http://ntour.ntou.edu.tw/handle/987654321/27031
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

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