Please use this identifier to cite or link to this item:
|Title: ||DNA mismatch binding activities in Chlorella pyrenoidosa extracts and affinity isolation of G-T mismatch binding proteins.|
|Authors: ||Todd Hsu;Kai-Ning Chang;Yi-Show Lai;Ting-Yi Jung;Gen-I Lee|
|Contributors: ||NTOU:Institute of Bioscience and Biotechnology|
|Keywords: ||Affinity adsorption;Binding proteins;Chlorella pyrenoidosa;DNA mismatch;G-T mispair|
|Issue Date: ||2011-10-21T02:22:21Z
|Publisher: ||Plant Physiology and Biochemistry|
|Abstract: ||Abstract:DNA mismatch recognition proteins contained in the extracts of unicellular alga Chlorella pyrenoidosa were isolated by affinity adsorption and 2-D gel electrophoresis. Incubation of the algal extracts with a 38-mer duplex oligonucleotide carrying a single DNA simple mispair generated a few gel retardation complexes. G-T mispair was recognized significantly better than C-T, G-G, G-A, and C-C mispairs by the algal extracts and these extracts bound very weakly to G-A and C-C mispairs, displaying a universal trend of mismatch binding efficiency. The levels of mismatch recognition complexes were slightly increased in the presence of 1 mM ATP. Two 13-kDa G-T binding polypeptides possessing pIs of 5.3 and 5.5 were isolated after resolving affinity-captured proteins by 2-D gel electrophoresis and the two factors were found to bind 5.5- and 2.8-fold stronger to heteroduplex than to homoduplex DNA, respectively. No proteins significantly homologous to the two algal G-T binding proteins were found by peptide mass fingerprinting (PMF). The sequence of a peptide generated from trypsin-cleavage of one G-T binding factor (pI 5.5) could be aligned with the amino acid sequences that form the C-terminal active sites of human and mouse mismatch-specific uracil/thymine-DNA glycosylases, suggesting the possibility of this factor as an algae- or a Chlorella-specific DNA mismatch glycosylase.|
|Relation: ||43(4), pp.309–313|
|Appears in Collections:||[生命科學暨生物科技學系] 期刊論文|
Files in This Item:
There are no files associated with this item.
All items in NTOUR are protected by copyright, with all rights reserved.